Abstract

A new electrochemical sensor based on a Nafion, aminated reduced graphene oxide and chitosan functional membrane-modified glassy carbon electrode was proposed for the simultaneous detection of adenine and guanine. Fourier transform-infrared spectrometry (FTIR), transmission electron microscopy (TEM), and electrochemical methods were utilized for the additional characterization of the membrane materials. The prepared electrode was utilized for the detection of guanine (G) and adenine (A). The anodic peak currents to G and A were linear in the concentrations ranging from 0.1 to 120 μM and 0.2 to 110 μM, respectively. The detection limits were found to be 0.1 μM and 0.2 μM, respectively. Moreover, the modified electrode could also be used to determine G and A in calf thymus DNA.

Highlights

  • Adenine (A) and guanine (G) are vital constituents of deoxyribonucleic acids

  • Study, a and new electrochemical based membrane on Nafion (NF), graphene chitosan (CHT)sensor functional was aminated proposed reduced for the graphene oxidedetection (ARGO) and functional membrane was proposed for the simultaneous simultaneous of Achitosan and G.(CHT)

  • The transmission electron microscopy (TEM) images of ARGO, CHT-ARGO, and NF-CHT-ARGO are shown in Scheme 1a–c, respectively

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Summary

Introduction

Adenine (A) and guanine (G) are vital constituents of deoxyribonucleic acids. They are very important in storing genetic information. 3 4 nanotubular-carboxyl-functionalized multi-carbon nanotubes composites [24], and bimemtallic properties. Recently,[25,26], graphene oxide, nanofibers, fullerenes, carbon better nanotubes, and other carbon. Graphene oxide, nanofibers, fullerenes, carbon and otherofcarbon allotropes allotropes have become important research objects for nanotubes, the improvement electrochemical have becomesystems. A new electrochemical sensor based on Nafion (NF), aminated reduced. Study, a and new electrochemical based membrane on Nafion (NF), graphene chitosan (CHT)sensor functional was aminated proposed reduced for the graphene oxidedetection (ARGO) and functional membrane was proposed for the simultaneous simultaneous of Achitosan and G.(CHT). A and ARGO is a reduced graphene covalently piperazine, and is piperazine, is G. homogeneously dispersed in theoxide membrane. The functional membrane-modified properties forelectrode the measurement of G and Aelectro-catalytic (Scheme 1).

Reagents
Instruments and Detections
Preparationof the Calf Thymus DNA Sample
HPLC Analysis on the Real Sample
Microscopic and Structural Studies
Characterization
Cyclic
Effects value electrochemical oxidation μM
Potential
Conclusions

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