Abstract
An indirect ELISA has been developed successfully for the detection of defined amounts of goats’ milk (1–100%) in ewes’ milk. The assay uses polyclonal antibodies against goats’ whey proteins (GWP) raised in rabbits. The anti‐GWP antibodies were recovered from the crude antiserum by immunoadsorption and elution from a column containing immobilized GWP. The anti‐GWP antibodies were biotinylated and rendered goats’ milk‐specific by mixing them with lyophilized cows’ and ewes’ whey proteins. ExtrAvidin‐peroxidase was used to detect the specific anti‐GWP antibodies bound to goats’ milk proteins immobilized on 96‐well plates. Subsequent enzymic conversion of substrate resulted in discernible differences in optical density between mixtures of ewes’ milk containing variable amounts of goats’ milk.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
