Abstract
Background:Cervical cancer is one of the most significant cancer found in women worldwide especially in developing countries. Previous reports showed that global DNA hypomethylation was correlated with various types of cancer including cervical cancer. Methods:Long interspersed nuclear element-1 (LINE1) pyrosequencing and Enzyme linked-immunosorbent assay (ELISA) assays were used for detection of global DNA methylation. The ELISA results were compared to bisulfite LINE1 pyrosequencing assay. Results:Different cervical cancer cell lines (CaSki, SiHa, HeLa, ME180, MS751, C33A) showed low global methylation percentage when compared to normal white blood cells by ELISA assay (1.47%-5.09% vs 8.20%, respectively) and by LINE1 pyrosequencing (20%-45% vs 62%, respectively). Global DNA methylation levels in cervical cancer samples were lower than precancerous lesions (Normal-CIN3) by LINE1 pyrosequencing (mean, 48.8% vs 56.9%, respectively, p<0.05) and ELISA assay (mean, 3.03% vs 3.85%, respectively, p<0.05). Conclusion:Global DNA hypomethylation was predominantly found in cervical cancer samples detected by ELISA and LINE1 pyrosequencing assays and could be used as triage tests in cervical cancer screening. ELISA assay is a suitable method for detection of global DNA methylation in large population; however, it should be further evaluated in a large clinical samples in order to be used as screening method.
Highlights
Cervical cancer is one of the most common cancer found in women worldwide, approximately >500,000 cervical cancer cases were diagnosed annually and nearly 50% death (Torre et al, 2017)
The results of global methylation percentage by Enzyme linked-immunosorbent assay (ELISA) found in various cervical cancer cell lines infected with different human papillomavirus (HPV) types were as followed, 3.72% (Caski), 1.74% (SiHa), 2.42% (HeLa), 4.79% (ME189), 5.09% (MS751) and 1.47% (C33A) while DNA extracted from normal white blood cells showed 8.20%
Methylation levels in clinical samples 167 of 280 cervical samples were available for global DNA methylation by pyrosequencing and means of methylation were as followed; 55.6% of normal, 56.8% of CIN1, 57.3% of CIN2-3 and 48.8% of cervical cancer, there was a significant difference between cervical cancer samples and precancerous samples (p
Summary
Cervical cancer is one of the most common cancer found in women worldwide, approximately >500,000 cervical cancer cases were diagnosed annually and nearly 50% death (Torre et al, 2017). Methods: Long interspersed nuclear element-1 (LINE1) pyrosequencing and Enzyme linked-immunosorbent assay (ELISA) assays were used for detection of global DNA methylation. Results: Different cervical cancer cell lines (CaSki, SiHa, HeLa, ME180, MS751, C33A) showed low global methylation percentage when compared to normal white blood cells by ELISA assay (1.47%-5.09% vs 8.20%, respectively) and by LINE1 pyrosequencing (20%-45% vs 62%, respectively). Global DNA methylation levels in cervical cancer samples were lower than precancerous lesions (Normal-CIN3) by LINE1 pyrosequencing (mean, 48.8% vs 56.9%, respectively, p
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
