Abstract
Two commercially produced enzyme-immunosorbent assays (EIAs) to detect antigens ofGiardia lambliain stool specimens, Ridascreen Giardia and Serazym Giardia, were evaluated. A total of 88 stool specimens were collected from patients who presented for various medical complaints in our outpatient clinic. Every specimen was examined by a conventional microscopic examination (CME), PCR and tested by both EIA kits. When microscopy was used as the reference standard, the Ridascreen Giardia showed a sensitivity of 72.9% and a specificity of 100%. Serazym Giardia had a sensitivity of 93.8% and a specificity of 100%. Antigen detection by EIA has been established as a valuable tool to make parasite stool diagnostics more effective.
Highlights
The 88 specimens were examined by conventional microscopic examination (CME), immunofluorescence microscopy (IFM), polymerase chain reaction (PCR) and two enzyme-immunosorbent assays (EIAs)
With PCR used as a background for the EIAs, results became more divergent (Table 3): Ridascreen Giardia performed with a sensitivity of 73.2% and a specificity of 83.4%, while Serazym Giardia showed a sensitivity of 95.1%, and a specificity of 87.2%
We evaluated the performance of two commercially available EIA kits for detecting Giardia lamblia antigens
Summary
EIA results were compared with those obtained by conventional microscopic examination (CME), immunofluorescence microscopy (IFM) and by PCR. Enzyme immuno-assays (EIAs) for detection of the specific antigens in stools have developed into an efficient diagnostic technique in the detection of Giardia lamblia[4,7,8,9]. In this study we tested two commercially available EIA kits that detect antigens of Giardia lamblia in stool samples (Ridascreen Giardia; manufactured by r-biopharm, and Serazym Giardia; manufactured by Seramun Diagnostica).
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