Abstract
A filtration procedure was devised to recover Giardia cysts from water using 5 μm Nuclepore membranes (110 mm in dia). Cysts taken from a beaver ( Castor canadensis) were added to 100 l. of untreated stream water and recovered by filtration. Cysts were washed from the membrane, concentrated by centrifugation and microscopically examined. Recovery efficiencies averaged 53% at cyst concentrations between 0.5 and 45 cysts l −1. Maximum cyst recovery was observed at filtration pressures of 40–60 kPa. The advantages offered by this method over cartridge filtration methods include higher recovery efficiencies at low cyst concentrations and simpler, more rapid laboratory procedures.
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