Detection of genetic variation in sandalwood using various DNA markers.

Dimple M Patel,Jigar G Mistry,Amar A Sakure,Sushil Kumar,Mukesh Kumar,Ranbir S Fougat

doi:10.1007/s13205-016-0391-0

Dimple M Patel, Jigar G Mistry + Show 4 more

Open Access

https://doi.org/10.1007/s13205-016-0391-0

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Journal: 3 Biotech	Publication Date: Feb 13, 2016
Citations: 30	License type: CC BY 4.0

Affiliation: Anand Agricultural University

Abstract

In the present study, 20 sandalwood (Santalum album L.) genotypes were characterized using RAPD, ISSR and SSR markers. Twenty-five RAPD and twenty-one ISSR primers that generated clear and reproducible banding patterns amplified 225 and 208 bands, respectively, among 20 sandalwood genotypes. Out of 225, 181 (83.13 %) RAPD bands were polymorphic while out of 208, 156 (75.77 %) ISSR bands were polymorphic. The average polymorphism information content (PIC) for RAPD and ISSR was 0.84 and 0.86, respectively. A good correlation (0.96) was observed between the matrices produced by RAPD and ISSR primers. Though, there was high similarity among genotypes (0.79 for RAPD and 0.70 for ISSR), the observed genetic diversity was found good enough for the characterization of sandalwood genotypes. Cross-species transferability SSR markers developed in S. austrocaledonicum and S. insulare were found to be monomorphic. The results of the present investigation would provide valid guidelines for collection, conservation and characterization of sandalwood genetic resources.

Highlights

Sandalwood (Santalum album L; 2n = 20) is one of the important tropical trees which is commercially known for its fragrance (Shashidhara et al 2003)
S. album or Indian sandalwood is of great commercial value due to its fragrant heartwood which yields unique oil preferred for perfumeries, cosmetics, medicines and in incense sticks industries
Overexploitation of forests for commercial purposes and other developmental activities have resulted in serious threat to tree species including sandal wood

Summary

Introduction

Sandalwood (Santalum album L; 2n = 20) is one of the important tropical trees which is commercially known for its fragrance (Shashidhara et al 2003). With high economic value of sandalwood and its oil, sandalwood wealth in forests are declining due to overharvesting and illegal poaching in natural habitats (Naseer et al 2012). This alarming genetic erosion condition indicates that there is need to conserve this commercially important tree species. To examine the existing genetic variability, there is urgent need for systematic variability study in sandalwood

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Conclusion