Abstract
Genetic diversity of Sri Lankan goats (Capra hircus) was evaluated using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method to detect polymophism in four candidate genes (LALBA, k-CSN3, GnRHR and BMP4) in a sample of 219 goats representing three phenotypic groups. All studied loci were polymorphic, having three morphs for BMP4, LALBA and two morphs for k-CSN3 and GnRHR in the study population. A significant difference between genotypic frequencies for BMP4 and GnRHR genes was found while PIC criterion revealed an intermediate polymorphism for all analyzed phenotypic groups except for k-CSN3 and GnRHR genes. Cross-bred animals for BMP4 gene and nondescript animals for LALBA gene were comparatively more polymorphic according to the effective allele number criterion. This study provides insight into the genetic diversity of Sri Lankan goats, which can be utilized to develop single nucleotide polymorphism markers to be used in association studies, and marker assisted selection.Int J Appl Sci Biotechnol, Vol 4(1): 133-138
Highlights
The studies on genetic diversity allow the genetic characterization of desired breeds and documentation of correlations between genotypic variations and related phenotypic traits
We present the genetic diversity of four candidate genes (LALBA, k-CSN3, Gonadotropin Releasing Hormone Receptor (GnRHR) and Bone Morphogenetic protein 4 (BMP4)) detected utilizing Single Strand Conformational Polymorphism (SSCP) in goats of Sri Lanka
PCR amplification of LALBA, k-CSN3, BMP4 and GnRHR was done as described by Jain et al, 2009; Kiplagat et al, 2010; Fang et al, 2009 and Yang et al, 2011 respectively
Summary
The studies on genetic diversity allow the genetic characterization of desired breeds and documentation of correlations between genotypic variations and related phenotypic traits. We present the genetic diversity of four candidate genes (LALBA, k-CSN3, GnRHR and BMP4) detected utilizing Single Strand Conformational Polymorphism (SSCP) in goats of Sri Lanka. Sample Collection Altogether 219 goats representing phenotypically nondescript, cross-bred and pure Jamnapari animals were DNA amplification by PCR Four gene fragments representing four candidate genes (LALBA, k-CSN3, GnRHR and BMP4) of the Capra hirucs genome were amplified.
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