Abstract
AbstractThe randomly amplified polymorphic DNA (RAPD) technique was used to investigate the genetic diversity in 6 strains of Xanthomonas campestris pv. mangiferaeindicae (Xcmi), the causal pathogen of mango bacterial canker disease (MBCD). The RAPD analysis was also intended to identify molecular markers, specific to the species to develop PCR-based markers for detection of Xcmi in mango field and planting materials. Twenty RAPD primers (CP 1-CP 20) were evaluated to establish molecular characters and genetic variability in the genome of Xcmi. Among these, only 4 were found efficient for development of reproducible banding pattern. It has been observed that the largest and smallest amplified RAPD products were of 2.036 and 0.201 kbp. A total of 136 bands were scored against 6 strains of Xcmi. There was 7.66 per cent polymorphism in individual isolates which indicates significant polymorphism among the evaluated strains, with mean difference of 0.33 (Xcmi 2 vs. Xcmi 8) and 0.29 (Xcmi 10 vs. Xcmi 12). However, the single linkage euclidean distances were statistically significant (P>0.05), i.e., 0.58. The markers CP 5, 10, 16 and 19 were amplified in all the strains with polymorphic alleles, which indicates that these markers could be used for rapid detection of genetic variability in Xcmi strains.
Highlights
Mango (Mangifera indica L. ) suffers from a number of diseases caused by bacteria, fungi and other agents
The randomly amplified polymorphic DNA (RAPD) technique was used to investigate the genetic diversity in 6 strains of Xanthomonas campestris pv. mangiferaeindicae (Xcmi), the causal pathogen of mango bacterial canker disease (MBCD)
DNA-based markers is generally determined by the technology, used to reveal DNA-based polymorphism through PCR (Williams, et al, 1990) with random primers can be used to amplify a set of randomly distributed loci in any genome and facilitated the applicability, i.e., the RAPD technique
Summary
Mango (Mangifera indica L. ) suffers from a number of diseases caused by bacteria, fungi and other agents. Title: Detection of genetic diversity among Indian strains of Xanthomonas campestris pv. Abstract The randomly amplified polymorphic DNA (RAPD) technique was used to investigate the genetic diversity in 6 strains of Xanthomonas campestris pv. Mangiferaeindicae (Xcmi), the causal pathogen of mango bacterial canker disease (MBCD).
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