Detection of Genes Responsible for Resistance to Quinolones Antibiotic in Salmonella Enterica Using the Polymerase Chain Reaction (PCR)

Abstract

Standard microbiological procedures were used for the isolation and identification of the Salmonella enterica isolates. Salmonella strains resistant to quinolone antibiotics were tested and screened for according to CLSI standards for antibiotic susceptibility testing. employing qnrA, qnrB, and qnrs-specific primers in polymerase chain reaction (PCR) to identify qnr genes. The findings showed that 22 strains (59%) contained the qnr S gene, found that both the qnrA and qnrS genes were present in 7 strains (18%) of Salmonella. Two isolates (5%) had qnrB gene positive tests, while 11 strains did not have any stribany qnr genes at all. This approach allowed us to verify that the Quinolones genes are diluted throughout the examined salmonella strains.