Abstract
Introduction. Silver nanoparticles are used in endodontics due to their antimicrobial activity, although it is considered that bacteria are unable to develop resistance to silver nanoparticles. Silver resistance genes have been related to resistance to nanoparticles and antibiotics. The presence of these resistance genes has not been studied in endodontic bacteria. The objective of this study is to report the prevalence of silver resistance genes in endodontic bacteria. Methods. The selected teeth were isolated using a rubber dam and any restoration, post, or caries was eliminated. The operative field was disinfected, and the root-filling material was removed. The samples were obtained using three sterile paper points to absorb the fluid of the root canal. The DNA from the samples and the control organism was extracted, and the detection of the silCBA resistance genes was carried out by PCR. Results. The results of this study show a high prevalence (73.3%) of silCBA silver resistance genes. The Spearman rank correlation coefficient was utilized to identify correlations between the presence of genes and clinical variables. Conclusions. This study reports a high frequency of silver resistance genes related to nanoparticle resistant from bacteria.
Highlights
Silver nanoparticles are used in endodontics due to their antimicrobial activity, it is considered that bacteria are unable to develop resistance to silver nanoparticles
This study demonstrates a high prevalence of silver resistance genes in endodontic bacteria
This could indicate that endodontic bacteria are able to develop resistance to silver nanoparticles in the long term. This invites us to reevaluate the use of nanoparticles as antimicrobial agents in endodontics, since the increase in this type of resistance could bring serious side effects such as costimulation of the increase in resistance to antibiotics and other antimicrobial agents, which would make the management of resistant infections even more difficult
Summary
Silver nanoparticles are used in endodontics due to their antimicrobial activity, it is considered that bacteria are unable to develop resistance to silver nanoparticles. Silver resistance genes have been related to resistance to nanoparticles and antibiotics The presence of these resistance genes has not been studied in endodontic bacteria. The objective of this study is to report the prevalence of silver resistance genes in endodontic bacteria. The results of this study show a high prevalence (73.3%) of silCBA silver resistance genes. This study reports a high frequency of silver resistance genes related to nanoparticle resistant from bacteria. The plasmid was named pMG101, and it confers resistance to Ag+, Hg+, and tellurite as well as antibiotics, such as ampicillin, chloramphenicol, tetracycline, and streptomycin This plasmid contains a region of 14.2 kb (sil operon) with nine ORFs (open reading frames) arranged in three transcriptional units (silCFBAGP, silRS, and silE) expressed from a different promoter. This transcription unit encodes an internal membrane protein complex formed by three
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
