Abstract
We used principal component analysis to develop measures (called Z-parameters in this study) which reflect the diversity of codon usage in Escherichia coli genes. Protein production levels for 1500 CDSs (protein-coding sequences) identified by E.coli genome projects in Japan and the US were estimated from a correlation equation between Z1 and cellular protein content obtained through analysis of the genes experimentally characterized. Through the profile analysis of Z1 for E.coli sequences obtained by the Japanese Project, we predicted an additional 36 CDSs that had not been annotated in the International DNA Database. Thirty-one out of the 36 CDSs could be assigned to presumptive protein genes through a BLASTX search for recent protein databases in the Genome Net in Japan. Detailed examination of the Z1-parameter profile led us to assess sequencing errors which cause frame-shift.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
