Abstract
The guanine rich locations are present in human genome. Previous studies have shown that the presence of G rich sequences and motifs may be significant for gene activity and function. We decided to focus our interest to identify G rich motifs in promoters of oncogenes and tumor suppressor genes. We used a set of 100 most common oncogenes and tumor suppressor genes (TSG) for this analysis. We collected 600nt long promoters with -500 and +100 TSS (transcription start site) from the oncogenes and TSG set. Using a computer program, we calculated the G densities using numbers and locations of G forms with 100nt moving widow. We included G numbers from 2 to 7 guanines. Analysis shows that G density increases from -500 to +100 and more from TSS. G density is found to be maximum within -/+100 of TSS. The results of G densities were compared with the expression data of the selected oncogenes and tumor suppressor genes in patients with colon cancer (n=174).
Highlights
The guanine rich region is a relatively unexplored part of the human genome
The aim of this study is to find repeating G motifs consisting of 2, 3, 4, 5, 6, and 7 guanines in the promoter sequence of selected genes important for carcinogenesis (50 tumor suppressor genes and oncogenes)
The names of the oncogenes and tumor suppressor genes (TSG) most related to colon cancer are taken from Genecards [21]
Summary
The guanine rich region is a relatively unexplored part of the human genome. There are some algorithms to detect special motifs, such as G quadruplex, the algorithms to detect other types of G rich motifs do not exist. It was first reported in 1910 that guanylic acid forms a gel at high concentrations [1]. It is suggested that G-rich sequences may form some other structures. The presence of G-rich sequences is found in functional regions of many genomes. Occurring ‘G’ rich sequences, via non-Watson-Crick base pairing capable of forming Gquadruplexes and stabilized by cyclic Hoogsteen hydrogen bonding, have been implicated in some different genomic activities such as: transcription pausing, FMRP binding, mRNA stability, translation initiation as well as repression [3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
