Detection of free fatty acids following a conditioned taste aversion in rats

Abstract

A gustatory transduction mechanism for free fatty acids (FFAs) has been described in isolated rat taste receptor cells; however, the ability of behaving rats to detect FFAs has not been characterized. Through conditioned taste aversion (CTA) methodology, this study defines the ability of rats to detect and avoid the two principal FFA components of corn oil, linoleic and oleic acid. Following taste aversion conditioning, rats avoided both linoleic and oleic acid at greater than or equal to 66 μM and failed to avoid either 44 μM linoleic or oleic acid. Rats demonstrated generalized avoidances between 88 μM linoleic and oleic acid irrespective of presenting the FFAs as either unesterified acids dissolved in 5 mM ethanol or aqueous sodium salts, sodium linoleate and sodium oleate. Following a CTA to linoleic acid, rats did not show generalized avoidance of NaCl or ethanol, two potentially concomitant tastants in the oral cavity. A CTA to linoleic or oleic acid did produce a generalized avoidance to the other FFA. These results support the ability of rats to detect linoleic and oleic acid (> 44 μM) and suggest that the two FFAs share common orosensory properties. Furthermore, it is unlikely that the detection of the FFAs is due to an enhancement of other concomitant tastants such as saliva or the delivery solution.