Abstract
A liquid chromatographic method for the selective detection of riboflavin (vitamin B 2) and flavin adenine dinucleotide (FAD) utilizing a thin-layer amperometric detector with two electrodes in series is described. The upstream electrode was held at −0.4 V (vs. SCE) with the downstream electrode at +0.1 V (vs. SCE). The linear ranges are 40 ng−10 μg for riboflavin and 200 ng−8 μg for FAD and the limits of detection are 8 and 40 ng, respectively. The interference of thirteen different vitamins on the spectrophotometric or electrochemical detection of FAD and vitamin B 2 was studied and no interference was found using the two-electrode detector. The effects of light on riboflavin and FAD is discussed. The method is convenient, rapid and economic, and has high selectivity.
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