Abstract

Fibrin plates containing small quantities of plasminogen have been used to detect protein zones containing staphylokinase after isoelectric focusing in polyacrylamide gels. Staphylokinase promotes the conversion of plasminogen to plasmin, which in turn degrades fibrin so that clear zones are produced in an opaque fibrin plate. The result is conveniently recorded by photography. General aspects of this procedure and conventional zymogram techniques in connection with isoelectric focusing are discussed. It is suggested that studies of this type should be well fitted for investigations on multiple molecular forms of proteins.

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