Detection of Fibrinogen and Coagulation Factor VIII in Plasma by a Quartz Crystal Microbalance Biosensor

Chunyan Yao,Weiling Fu,Ling Qu

doi:10.3390/s130606946

Abstract

A quartz crystal microbalance (QCM) biosensor with nanogram sensitivity has been constructed through a reasonable designing and biological processing of the piezoelectric quartz crystals. Due to its highly sensitivity, real time detection and low cost, the proposed QCM biosensor has a promising potential in blood coagulation research. In the current study, the QCM biosensor was used to determine the activated partial thromboplastin time (APTT) for 120 anticoagulated plasma specimens. A good linear relationship was found in a double-logarithmic plot of APTT versus fibrinogen concentration in the range of 1.58–6.30 g/L. For factor VIII, the detection range by the QCM biosensor is 0.0185–0.111 mg/L. The QCM biosensor results were compared with those obtained by commercial optical coagulometry and a good agreement (correlation coefficient is 0.949 for fibrinogen, and 0.948 for factor VIII) was reached. Furthermore, the QCM determination can be completed within 10 min. Our study suggested that the proposed QCM biosensor could provide for more convenient and time saving operations, which may be useful in clinical situations for rapid monitoring of anticoagulant therapy using small volume (20 μL) plasma specimens.

Highlights

And reliable diagnosis of haemostatic disorders increases the possibility of successful medical treatment
Thrombin act on fibrinogens, fibrinogens decompose into peptide compounds and fibrin, fibrins gather and form insoluble clots
Making use of the special properties of quartz crystal microbalance (QCM) biosensor and the viscosity change of the coagulation reaction, we proposed a new strategy for fast detection of fibrinogen and coagulation factor VIII in plasma specimens

Summary

Introduction

And reliable diagnosis of haemostatic disorders increases the possibility of successful medical treatment. The analysis of blood coagulation factors is widely performed in the clinical laboratory because it plays a central role in providing important information to the physician so as to minimize the patient’s risk. Among these tests, fibrinogen and blood coagulation factor VIII (FVIII) are often analyzed in clinical laboratories to monitor the extent of the introduced anticoagulation. Fibrinogen, which plays an important role in the blood coagulation cascade, can be activated by thrombin and polymerized insoluble fibrin, a main component of clots [1]. Low plasma levels of coagulation FVIII leads to a tendency towards clotting inefficiencies, whereas high plasma levels of coagulation FVIII can result in various thrombotic diathesis, such as deep vein thrombosis [4,5,6]

Methods

Results

Conclusion