Abstract
Microscopic examination of 219 indirect anti‐human globulin tests using a 1: 1,000 dilution of RhoGAM®* and 259 indirect antihuman globulin tests using appropriate slide test anti‐Rho(D) sera on specimens simulating fetal maternal hemorrhages (FMHs) of from 15 to 200 ml showed that agglutination was more frequently observed using the slide test anti‐Rho(D) sera. Tests done on coded specimens sent to several hospital laboratories indicated the need for technologist education in the interpretation of results of these tests on postpartum women. Since the amount of Rh immune globulin needed to treat women with FMHs depends on the volume of fetal bleed, suspected FMHs should be confirmed and quantitated using Kleihauer‐Betke smears.
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