Abstract
The aim of this study was to investigate the presence of Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli strains isolated from meat and faecal samples of chicken. Faecal samples (n: 384) were collected from broiler and egg-type healthy chickens in 43 flocks and 384 fresh chicken meat samples from local markets, supermarkets and slaughterhouses. E. coli isolates were tested phenotypically according to the guidelines of CLSI (2013) for ESBL production. Phenotypic detection of AmpC production was carried out by determination of resistance to cefoxitin and susceptibility to cefepime. Also, presence of genes encoding different types of β-lactamases (blaCTX-M, blaTEM, blaSHV and the mutation in the promoter of AmpC) in all phenotypically ESBL or AmpC producing E. coli was investigated by PCR assays. In this study, blaCTX-M (95.4%), blaCTX-M-1 (81.8%), blaCTX-M-8 (4.5%), blaTEM (45.4%) and blaSHV (2.2%) genes were detected from faecal samples and blaCTX-M (100%), blaCTX-M-1 (28.5%) and blaTEM (7.1%) from meat samples. The only plasmidic AmpC β-lactamase found was the CIT type.
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