Abstract
Escherichia coli O157:H7 has been identified as a causative agent in outbreaks of hemorrhagic colitis and hemolytic uremic syndrome involving ground beef and apple cider. A microplate sandwich immunoassay for the detection of E. coli O157:H7 from food was developed utilizing time‐resolved fluorometry. Biotin‐labeled, polyclonal anti‐E. coli O157:H7 antibodies immobilized on streptavidin‐coated microtiter plates were used to capture the bacteria. The bound antigen‐antibody complex was then detected using the same antibodies labeled with europium chelates. The effects of capture antibody concentrations, immobilization procedures, and addition of 0.1% Tween 20 to assay buffer were studied during assay development. The detection threshold for the assay developed is 103‐104 CFU/mL. The optimized assay was further tested in ground beef and apple cider samples spiked with E. coli O157:H7. The detection limit was < 10 CFU/g of ground beef and 10 CFU/mL of apple cider with 6 h of enrichment.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
