Abstract
The Japanese entomopathogenic fungus Entomophaga maimaiga can be detected in infected gypsy moth larvae, Lymantria dispar, using an indirect enzyme-linked immunosorbent assay (ELISA). Polyclonal antibodies specific to the plasma membranes of the protoplast stage of E. maimaiga (the fungal stage within the hemolymph of infected insects) were used for ELISA development. Fungal infection can be detected 3 days after conidial inoculation with 71.1% accuracy and after 4 days with 97.8% accuracy. Antibodies do not cross-react with the gypsy moth nucleopolyhedrosis virus or four North American endemic species of entomopathogenic fungi known to infect the gypsy moth. One isolate in the same phylogenetic grouping as E. maimaiga, the E. aulicae species complex, is detectable using E. maimaiga antisera.
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