Detection of enterovirus in CSF of patients with sporadic infection of CNS

Abstract

Objective We detected the enterovirus in the CSF of patients with sporadic aseptic meningitis and viral encephalitis in to investigate the enterovirus infection in this area, and two methods were used in this study. Methods CSF samples of 36 patients with sporadic aseptic meningitis and viral encephalitis were collected during 2009-2013. All the samples were frozen and stored at -80℃ immediately before RNA extraction. Total viral RNA extraction was taken within 3 month after the sample was stored by using an RNA extraction kit according to the method described by the instruction of the kit.Then RNA was reversed into cDNA immediately after the extraction of RNA. cDNA was stored at -80℃. Finaly, we used a rapid and sensitive method for the detection of all members of the enterovirus genus by using real-time PCR. We also detected the IgM of 7enterovirus including Echo virus and Coxsackie virus 1-6 serotype by Enzyme-Linked Immunosorbent Assay(ELISA) method. Results Of all the 36 CSF samples, 9(25%) were positive for enterovirus by PCR method. For patients with aseptic meningitis, viral encephalitis and meningoencephalitis, the proportion were 42.86%, 12.50% and 11.11%. Only 2 samples were positive for Coxsackie virus by ELISA method, and both of them had aseptic meningitis. Conclusion Enterovirus infection is a common cause of sporadic CNS virus infection among patients with viral encephalitis, aseptic meningitis and aseptic meningoencephalitis in Beijing, especially for the patients with aseptic meningitis.Real-time PCR was more sensitive than ELISA, while the better way is to use both of them to detect the serotype of the enterovirus. Key words: Enterovirus; Meningitis; Encephalitis; Infection