Abstract
The current work was performed to detect Staphylococcus aureus and their enterotoxin genes as sea, seb, sec andsed among street vended meat products represented by beef burger, kofta and sausage (30 of each) using traditional method of bacteriological isolation and multiplex PCR. The results illustrated that 37 (41.11%) out of 90 meat product samples harbored S.aureuspathogen. Accurately, S.aureus enterotoxin genes were detected in 13 (35.1%) out of 37 isolates. 3 isolate for beef burger samples mostly harboured (2) sea and (1) seb and the other 6 strains was negative for classic enterotoxin genes. Meanwhile, 5 isolates for kofta samples harboured (2) sea, (1) sec, (1)sedand(1) sea&sedgene and the other 7 were negative for classic enterotoxin genes, but 16 isolates of sausage samples harboured (2) sea, (1) seb, (1) mixed seb&sec and (1) mixed sea, sec & sec genes and 11 was negative for classic enterotoxin genes. Furthermore, seais more prevalent in 6 samples (16.2%) followed by sebin 2 samples (5.4%) and sec, sed, sea &sed, seb&secand sea &sec&sed in one sample (2.7%). Finally, Multiplex PCR is efficient in detection of SEs in food. Contamination of meat products with such serous pathogen was discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
