Detection of enterobacteria using polymerase chain reaction real-time

Abstract

The purpose of the work is to carry out a real-time polymerase chain reaction for the isolation of enterobacteria from the faeces of the maize snake and their identification.Fecal masses after molting were collected from a male maize snake named Rabbit of Bloodred het Pied-Sided morph.Egg release date: 06/19/2020.Isolation of nucleic acids from faecal samples was carried out by the sorption method using the AmpliPrime DNA-sorb-AM reagent kit (Central Research Institute of Epidemiology) in accordance with the manufacturer's instructions.For amplification, a test was used the Salm-IDS system for the detection of pathogenic Salmonella (Samonella spp et Salmonella Typhi). The amplification process was carried out in a LightCicler 96 (Roche) PCR-RT instrument.In the study of feces from a reptile by the molecular method, the genus Salmonella was discovered.The PCR study did not allow for species identification and determination of the viability of the microorganism, it is necessary to create a scheme for laboratory control of salmonella infection, which will include both the PCR method and bacteriological studies.