Abstract
The edible insects are commonly used in food products, but the methods for detection are not yet established. This study presents a new bright-field method for detecting insects in food using histochemical staining (PAS-Calleja and Pasini). For the purpose of the study, snack bars from house cricket (Acheta domesticus) and mealworm (Tenebrio molitor) were produced in the concentration range of 0.0–25.0%, and for the purpose of verification, also samples from the market network were obtained. A 100% consensus of evaluators on the presence of both insect species was demonstrated in the snack bars from the concentration of 2.5%. Sensitivity and specificity were 100%, reliability was 95.4% for PAS-Calleja staining For Pasini staining sensitivity was 93%, specificity was also 100% as PAS-Calleja staining and reliability was 94.2%. The cuticle appears to be crucial for evaluating the presence of insects due to its high affinity for the PAS-Calleja staining used. A homogeneity test was carried out within the evaluation. The distance between individual fragments of insects was statistically significant at low concentrations. In conclusion, it can be assessed that evaluators can confirm the positivity of a bar samples for the presence of insects (ACH and TM) from a 1% content using the PAS-C staining histochemical method.
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