Abstract
Highly sensitive labeled detection of Echinococcus granulosus using colloidal quantum dots (QDs) based on a porous silicon Bragg mirror sensor are demonstrated. Rabbit anti-p38 labeled CdSe/ZnS QDs was infiltrated in porous silicon pores immobilized Egp38 antigen. QD-antibodies are specifically bound to antigens linked covalently to the pore walls of PSi after the immune reaction. By the design of the transfer matrix method and the preparation of the electrochemical etching method, the fluorescence peak wavelength of the quantum dots is located in the forbidden band of the Bragg mirror. The fluorescence of QDs are enhanced by PSi Bragg mirror. Egp38 antigen detection limit of 300fg/mL is achievable. Our results exhibit that the biosensor combining PSi Bragg mirror and QDs can potentially be applied to the clinical detection of hydatid disease.
Highlights
IntroductionHydatid disease is a parasitic disease caused by Echinococcus granulosus ( known as tapeworm) that threatens both livestock and humans
Hydatid disease is a parasitic disease caused by Echinococcus granulosus that threatens both livestock and humans
The solution was centrifuged to remove any unreacted rabbit anti-p38, bovine serum albumin (BSA), ethyl-(3dimethylaminopropyl) carbodiimide hydrochloride (EDC), sulfo-NHS, and quantum dots (QDs) and the pellet containing QD-rabbit anti-p38 was dissolved in 600 μl phosphatebuffered saline (PBS) and stored at 4°C. 2.3 Fabrication of porous silicon (PSi) photonic device PSi photonic device with Bragg mirror is designed by the transfer matrix method
Summary
Hydatid disease is a parasitic disease caused by Echinococcus granulosus ( known as tapeworm) that threatens both livestock and humans. The first kind of detection is based on the measurement of refractive index change caused by biological reaction in PSi device. The biological reaction can be detected by measuring the reflection spectrum shift caused by the refractive index changes of these devices. A spectrometer-free method based on PSi microcavity was developed to measure the refractive index changes with goniometer by the angle spectrum of reflection light Using this method, label-free biological detection can be realized with high detecting resolution [12]. Based on the characteristics of the PSi Bragg mirror, we have achieved a highly sensitive biological detection by measuring the fluorescence intensity of QDs attached to the target molecules after biological reaction in this paper. Utilizing the QD/PSi optical biosensor, Egp antigen detection limit is 300fg/mL
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