Detection of Drug Resistant Elements from Broiler Feces Based on PCR Technology

Abstract

Objective: The aim of this study is to detect various drug resistance elements from chicken feces by PCR technology, so as to lay a foundation for the application of PCR technology in the survey of drug resistance of intestinal microbiota. Methods: Genomic DNA was extracted from chicken feces, and different drug resistance elements were amplified by conventional PCR. Results: In this study, different classes of the drug-resistant genes <i>tet</i>A, <i>tet</i>B, <i>bla</i><SUB>SHV</SUB>, <i>bla</i><SUB>TEM</SUB>, <i>bla</i><SUB>CTX-M1</SUB>, <i>qnr</i>A, <i>aad</i>A1, <i>dfr</i>A1 and <i>sul</i>1 were observed from collected chicken feces, and the detection rates of different drug-resistant genes varied based on time point at which the genes were amplified. Wherein, <i>tet</i>B progressively increased with the prolongation of feeding time, while <i>bla</i><SUB>TEM</SUB> and <i>aad</i>A1 decreased with the prolongation of feeding time. In addition, integrase genes <i>int</i>I1 and <i>int</i>I2 were also detected in this study. Among the types of plasmid replicons, IncB/O, IncFIC, IncFIA, IncFIB, IncI1, IncN and IncFrep were detected in this study, and the number of replicon types carried by different samples ranged from 1 to 6, and the detection rate of plasmid replicons also had a time point difference, while, obvious regularity was not observed. In terms of the correlations between plasmids, the detected plasmids presented varying degrees of correlations. Conclusion: PCR technology could detect different drug resistance elements in intestinal microbiota.