Detection of DNA mutation, urinary diseases and blood diseases using surface plasmon resonance biosensors based on Kretschmann configuration

Abstract

Surface plasmon resonance (SPR) is one of the most attractive recent methods for surface-sensitive bio-molecular interaction analysis (BIA) and detection of various phenomenon. Its key advantages include surface-sensitive response, label-free detection, and real-time measurement capability and ability to detect even minute changes. As a result, one of the priority directions in the SPR biosensor research has been the development of SPR based sensors for detection of specific biological and chemical analytes related to the application areas such as medical diagnostics, environmental monitoring, food safety and security. Specially, medical diagnostics has been perceived as one of the most significant field for applications of SPR biosensor technology. Therefore, SPR biosensors for detection of molecules related to medical diagnostics are currently being widely researched. Here, we present the analysis of detection of three potentially threatening health conditions namely early detection of DNA mutation and some urinary and blood diseases, based on SPR biosensors of Kretschmann configuration. Experimental values of refractive indices corresponding to various conditions have been used from literature and finite-difference time-domain simulations have been performed to obtain the angle at which minimum reflectance (R min ) occurs in an SPR sensor using Kretschmann configuration. The detection plausibility of varying normal as well as diseased cases has been analyzed observing the shifts in the resonance dip angle.