Abstract
In this study, the HAT-RAPD technique was used to detect DNA methylation in the four plant species, rice (Oryza sativa), petunia (Petunia hybrida), spinach (Spinacea oleracea L.) and longan (Dimocarpus longan Lour.), which were treated using 5-azacytidine, potassium chlorate (KClO3) and a low temperature induction. Rice and petunia were chosen to be induced because in these species it is known that 5-azacytidine is able to induce hypomethylation in their genomes leading to morphological changes, in particular floral induction, in the developing plants. Using the HAT-RAPD technique, DNA methylation was detected using the restriction enzymes HpaII and MspI in rice and petunia (as expected) and in spinach, but was found to be absent in longan, which suggests that floral induction in longan is undetectable in longan.
Highlights
Many plants in temperate zones require a low temperature stress to induce flowering and fruit production. In some plants such as Arabidopsis and Cichorium intybus the vernalization response has been linked to changes in DNA methylation[1,2], while the seedlings of other plants like Oryzia sativa are regularly treated with 5-azacytidine which results in dwarfism and a high tillering number due to hypomethylation of the cytosine nucleotide[3,4]
We investigated DNA methylation patterns in longan and spinach which were induced by 5-azacytidine, potassium chlorate (KClO3) and low temperatures, using the HAT-RAPD methodology
To test for the presence of DNA methylation dependent floral induction in the four plant species rice, petunia, longan and spinach, multiple treatment effects were tested in combination with a characterization of the corresponding phenotypic changes in each species
Summary
Many plants in temperate zones require a low temperature stress (vernalization) to induce flowering and fruit production. We investigated DNA methylation patterns in longan and spinach which were induced by 5-azacytidine, potassium chlorate (KClO3) and low temperatures, using the HAT-RAPD methodology
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