Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F 1 of Cultivated Rice ( Oryza sativa L.) Using SSR Markers

Abstract

S-a, S-b and S-c are three loci for F 1, pollen sterility in cultivated rice ( Oryza sativa L.). Taichung 65 (T65) is all S j/S j at these three loci, while its F 1 pollen sterile near-isogenic lines, TISL2 ( S-b), TISL4 ( S-a) and TISL5 ( S-c) is S i/S i according to their respective sterility locus. Using SSR molecular marker to detect the segregation of the allele S i and S j in pollen calli population induced from different hybrid F 1, which have different pollen sterility locus, showed that the segregation of allele S i and S j was distorted. The distorted direction of pollen calli population in vitro was not the same as F 2 population in vivo. The quantities of pollen callus carrying S j were much more than that of carrying S i at S-a and S-c locus, the ratio of S i and S j were 1:4.81 and 1:1.96 respectively. But the opposite tendency was observed at S-b locus, the ratio of S i and S j being 1:0.35. At the same time, all these results were undisturbed by either culture medium or culture period.