Abstract
Objective. To assess the presence of RNA from dengue virus by Real Time Polymerase Chain Reaction with Reverse Transcriptase (qRT-PCR), in anti-dengue IgM seronegative serum samples obtained from symptomatic patients with less than 5 days of fever, in the department of Bolivar, Colombia.Materials and Methods. Cross sectional descriptive study, where 50 anti-dengue IgM seronegative serum samples were analyzed by qRT-PCR. In addition, we reviewed the epidemiological charts of each patient in order to collect sociodemographic and blood count (CBC) data.Results. DENV-3 RNA was detected in seven (14Â %) serum samples. The most common clinical manifestations were: fever (90Â %; 45/50), headache (88Â %; 44/50) and myalgia (74Â %; 37/50). Regarding the CBC, the most significant findings were: thrombocytopenia (60Â %; 30/50; 73.3% were men), and leukopenia (50Â %; 25/50; 60% were men).Conclusion. Detection and typing of DENV genome by qRT-PCR could be considered a diagnostic tool for Dengue in anti-dengue IgM seronegative serum samples, especially, within four days after the onset of the signs of the disease.
Highlights
Dengue, the main arboviral infection in the world, is an acute viral disease epidemic in tropical and subtropical regions
Detection and typing of Dengue virus (DENV) genome by qRT-PCR could be considered a diagnostic tool for Dengue in anti-dengue IgM seronegative serum samples, especially, within four days after the onset of the signs of the disease
Serum samples were provided by the Bolivar Department of Public Health Laboratory, reference center in collecting samples from municipal hospitals for diagnosis of dengue virus
Summary
DENV-3 RNA was detected in seven (14 %) serum samples. The most common clinical manifestations were: fever (90 %; 45/50), headache (88 %; 44/50) and myalgia (74 %; 37/50). Regarding the CBC, the most significant findings were: thrombocytopenia (60 %; 30/50; 73.3% were men), and leukopenia (50 %; 25/50; 60% were men). Detection and typing of DENV genome by qRT-PCR could be considered a diagnostic tool for Dengue in anti-dengue IgM seronegative serum samples, especially, within four days after the onset of the signs of the disease. Dengue Virus; Dengue; Real-Time Polymerase Chain Reaction; RNA, Viral (source: MeSH, NLM)
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