DETECTION OF DENGUE TRANSOVARIAL VIRUS FOR AIDES AEGYPTI MOSQUITOES IN ENDEMIC AREAS OF DENGUE HEMORRHAGIC FEVER

Abstract

Background: The incidence rate (IR) of Dengue Hemorrhagic Fever per 100,000 population in Central Java has increased dramatically in five years. Banyumas Regency is an endemic area of ​​dengue hemorrhagic fever in Central Java Province. The cause of dengue hemorrhagic fever is four dengue viruses known as DEN-1, DEN-2, DEN-3, and DEN-4. The purpose of this research is to detect transovarial dengue virus in Aedes aegypti mosquito in Banyumas Regency.
 Methods: The study was conducted by installing ovitrap in Mersi Village, East Purwokerto District, and in Pliken Village, Kembaran District, Banyumas Regency. The study was conducted by installing ovitrap in Mersi Village (9 samples) and Pliken Village (3 samples). The eggs obtained are hatched into larvae in the laboratory. The 3rd and 4th instar larvae were examined for the presence of dengue virus using the Elisa method.
 Result: The results showed that in Mersi Village, there were two positive samples, and in Pliken Village, there was one positive sample containing dengue virus. The presence of the dengue virus found in the hatched Aedes aegypti larvae (F1) indicates that there has been a transovarial dengue virus from the parent mosquito in its offspring.
 Conclusion: The study concluded that in Banyumas District, a dengue virus transovarial had occurred in Aedes aegypti mosquitoes. The government and the community should increase efforts to eradicate mosquito nests (dengue hemorrhagic fever) to reduce the vector population of dengue hemorrhagic fever so that cases of dengue hemorrhagic fever can be eliminated. 
 
 Keyword: Transovarial dengue virus, Aedes aegypti, Dengue Hemorrhagic Fever