Detection of Deficient Erythrocyte Regeneration of Reduced Triphosphopyridine Nucleotide from Glucose-6-Phosphate

Abstract

Article1 May 1962Detection of Deficient Erythrocyte Regeneration of Reduced Triphosphopyridine Nucleotide from Glucose-6-PhosphateEvaluation of a Rapid Screening TestA. P. KRAUS, M.D., F.A.C.P., C. L. NEELY, M.D., F. T. CAREY, M.D., L. M. KRAUS, PH.D.A. P. KRAUS, M.D., F.A.C.P.Search for more papers by this author, C. L. NEELY, M.D.Search for more papers by this author, F. T. CAREY, M.D.Search for more papers by this author, L. M. KRAUS, PH.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-56-5-765 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe treatment of malaria by the 8-aminoquinoline compounds was accompanied by a small incidence of hemolytic anemia (1, 2) particularly in the pigmented races. Dernet al. (3), in 1954, utilizing chromium-51 tagged erythrocytes demonstrated the abnormality to be an intracorpuscular defect. Fegler (4) noted that erythrocytes did not hemolyzein vitrountil the reduced glutathione (GSH) fell below 40 mg per 100 ml red blood cells. Benesch and Benesch (5) demonstrated that blockage of erythrocyte sulfhydryl groups by arsenite resulted in hemolysis. Since glutathione constitutes the principal source of sulfhydryl groups in the erythrocyte, this finding suggested that it...