Abstract
We have studied the detection of cytochrome C by the use of surface plasmon resonance sensor on gold surfaces modified by alkyl thiols with different tail groups such as CH 3, NH 2 and CH 2OH. When the hexadecanethiol film was coated on gold surface, the incident angle changed from 23.1° to 53.2°. Moreover, the incident angle was changed from 53.2° to 63.6° by adding the phosphate buffer (PB) solution ( pH=7.06 ), followed by contacting the cytochrome C solution (10 μM) to 64.6°. The highest sensitivity was obtained on the thiol film having the hydrophobic group. The sensitivity decreased in the order CH 3> NH 2> CH 2 OH . As a result of examining the reproducibility, the incident angle returned at about 90% immediately, when the absorbed surface was washed in the imidazole solution (3 mM, pH=7.8 ).
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