Abstract
Detection of cyclic nucleotide phosphodiesterase mRNA in mouse skeletal muscle tissue and primary cultured myocytes
Highlights
The regulation of postnatal skeletal muscle development is complex, involving many integrated biochemical pathways that interact with the environment to control the rate of protein accretion
Hydrolysis of the cyclic nucleotides occurs at a rate 9-600-fold faster than the rate of cyclic nucleotide synthesis, suggesting that PDE plays the dominant role in the control of cyclic nucleotide levels in cells (Cheng and Boettcher, 1982)
We demonstrate for the first time the presence of mRNA for all PDE subtypes except PDE6A-6D in mouse skeletal muscle tissue and primary cultured myocytes
Summary
The regulation of postnatal skeletal muscle development is complex, involving many integrated biochemical pathways that interact with the environment to control the rate of protein accretion. At least 11 different PDE families (PDE1 to PDE11) have been identified in mammalian tissues on the basis of their amino-acid sequence homology and biochemical properties, including substrate specificity, stimulation or inhibition by endogenous regulators, and sensitivity to PDE inhibitors (Soderling and Beavo, 2000; Bingham et al, 2006). Of these eleven families, rolipram-sensitive PDE4, rolipram-insensitive PDE7 and PDE8 isozymes hydrolyse mainly cAMP. PDEs comprise a superfamily, and control cyclic nucleotide levels
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