Abstract
Background: Leishmaniasis is a parasitic diseases that are spread worldwide due to various species of Leishmania, which are infect mammales diversity as well as human. L. tropica, L. major, and L. aethiopica which is common causes of cutaneous Leishmaniasis in Salah Adeen and Baghdad provences.
 Material and Methods: The present study was conducted to investigate the prevalence of cutaneous Leishmaniasis and to identify Leishmania parasites by using polymerase chain reaction (PCR) in some endemic areas of Iraq. A total of 117 samples of patients with suspected cutaneous Leishmaniasis were collected in different age groups. And both sexes 73 male and 44 female patients.
 Results: PCR results showed the percentage of infections 62.39% of males while 37.60% of females. The average age was 23.35 years (the range, from 1- 60 years), with the highest percentage of cases in the age group 1-4 years and the lowest rate in the age group (40-60 years). The highest infection was by L.tropica of L.major and lowest infection caused by L.aethiopica, where is considered first revealed in Iraq.
 Conclusion: The study found that males were more likely to be infected than females. The study revealed that polymerase chain reaction (PCR) is the most effective and sensitive method for detecting types of Cutaneous Leishmaniasis.
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