Detection of Curlibiogenesis genes among Enterobacter cloacae isolates and their role in biofilm formation in Al-Hilla City, Iraq

Abstract

In this study, (100) clinical samples were collected from patients with urinary tract infection (UTI). Samples were then subjected for culturing on selective media to isolate Enterobacter cloacae. All clinical samples were positive culture, only (62) samples showed positive cultures, as gram negative bacteria whereas (38) bacterial growth were detected as gram positive bacteria. Among (62)gram negative bacteria, only 12(19.4%) isolates belonged to Enterobacter cloacae, isolates were identified by API20E. Sequencing of hsp60 gene and alignment of (341) base pare confirmed the identity of 8(12.9%) isolates. All DNA samples extracted from bacterial isolates were conducted for polymerase chain reaction to investigate presence of curli genes among isolates. The result revealed that 8(100%) of isolates were positive to Curli-specific Genes D(CsgD)gene, The Curli-specific Genes D (csgD)genes showed a bands of expected size (355) base pare in (8) isolates E. cloacae under study. Six of eight Enterobacter cloacae isolates produced (245) base pare amplification bands of Curli-specific Genes A (csgA)gene (75%). The results display significant positive correlation between biofilm formation and curlifambriae adhesion. Our results conclude the importance of curli fimbriae adhesion in establishment of biofilm and magnitude of biofilm formation in urinary tract infection (UTI)via antibiotics resistance and ascending infections.