Detection of Cryptosporidium spp in zoo of Mosul and Dohuk cities

W. A. Ahmed Al-Obaidi

doi:10.33899/ijvs.2006.45798

Abstract

This study includes detection of Cryptosporidium spp from fecal samples taken from different animals in Duhok and mosul Zoo , and parasite from workers in these Zoo in 2002 .The total infected percentage reach to (54.5 % , 46.6 % , 41.6 % , 80 % , 71.4, 60 %) in Monkeys, Deer, Parrot, Finch, Fox and Zoo Workers. Four laboratory method were used for diagnosing parasite oocysts, The results showed that efficient diagnostic method was modified acid fast stain method (100%), followed by Iodine staining method and flotation in saturated sugar solution method. While, formalin sedimentation method gave low confidence for diagnosis the oocysts of parasite in fecal samples. Features of oocysts were varied according to the technique was used for examination of fecal samples, but there are no morphological differences appeared in oocysts. The average of oocysts sizes were shown to be within the normal range of oocysts of Cryptosporidium spp. The average of oocyst sizes was shown to be within the measure of oocyst of Cryptosporidium spp.

Highlights

‫الخلاصة‬ ‫تضمنت ھذه الدراسة الكشف ع ن طفيل ي الاب واغ الخبيئ ة ‪Cryptosporidium spp‬‬ ‫في عين ات الب راز الم أخوذة م ن ع دد م ن الحيوان ات المرب اة ف ي ح دائق الحيوان ات لم دينتي‬ ‫الموصل ودھوك وكذلك الكشف عن وجود الطفيلي في عينات البراز للعم ال الق ائمين عل ى‬ ‫رعايتھ ا ف ي الح دائق ذاتھ ا خ لال ع ام ‪ .2002‬اس تخدمت ‪ 4‬م ن التقني ات المختبري ة ف ي‬ ‫التشخيص للكشف عن أكياس بيض طفيلي‪ Cryptosporidium spp‬حيث بلغت ن سبة‬ ‫الخم ج الكل ي ف ي الق رود‪ ،‬الغ زلان‪ ،‬الببغ اء‪ ،‬الف نجس والثعال ب )‪,%46.4, %54.5‬‬
Four laboratory method were used for diagnosing parasite oocysts,‬‬ ‫‪The results showed that efficient diagnostic method was modified acid fast stain‬‬ ‫‪method (100%), followed by Iodine staining method and flotation in saturated‬‬
Features of‬‬ ‫‪oocysts were varied according to the technique was used for examination of fecal‬‬ ‫‪samples, but there are no morphological differences appeared in oocysts

Summary

Introduction

‫الخلاصة‬ ‫تضمنت ھذه الدراسة الكشف ع ن طفيل ي الاب واغ الخبيئ ة ‪Cryptosporidium spp‬‬ ‫في عين ات الب راز الم أخوذة م ن ع دد م ن الحيوان ات المرب اة ف ي ح دائق الحيوان ات لم دينتي‬ ‫الموصل ودھوك وكذلك الكشف عن وجود الطفيلي في عينات البراز للعم ال الق ائمين عل ى‬ ‫رعايتھ ا ف ي الح دائق ذاتھ ا خ لال ع ام ‪ .2002‬اس تخدمت ‪ 4‬م ن التقني ات المختبري ة ف ي‬ ‫التشخيص للكشف عن أكياس بيض طفيلي‪ Cryptosporidium spp‬حيث بلغت ن سبة‬ ‫الخم ج الكل ي ف ي الق رود‪ ،‬الغ زلان‪ ،‬الببغ اء‪ ،‬الف نجس والثعال ب )‪,%46.4 , %54.5‬‬.

Results

Conclusion