Abstract
A simultaneous detection of penicillinase-producing Neisseria gonorrhoeae (PPNG) and non-penicillinase-producing N. gonorrhoeae (NNPNG) from 100 clinical swab samples was evaluated in a four-primer polymerase chain reaction (PCR). Target sequences were the cryptic plasmid and the TEM-1 gene, a β-lactamase coding gene. Compared with conventional gonococcal tests, results indicated that the PCR-based test is more sensitive and should play an important role as an alternative or adjunct to culture particularly in asymptomatic patients. It correctly identified four samples out of 100 which were positive by both microscopy and culture. It detected accurately penicillinase-producing from non-penicillinase-producing strains.
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