Abstract
Corynespora cassiicola, which causes Corynespora leaf fall disease, is one of the most destructive and economically important fungal pathogens of Hevea rubber trees in Asian and African countries. This fungus is present in some Hevea rubber plantations and nurseries in Hainan and Yunnan provinces of South China. We describe the development of a PCR assay for C. cassiicola. With this test, of the 32 leaf samples obtained from 28 Hevea rubber trees in 15 rubber plantations and nurseries of Hainan and Yunnan provinces, 21 tested positive for the fungus. This assay is useful for the screening and certification of young C. cassiicola-free Hevea rubber plants (for distribution to commercial growers) due to its high sensitivity, reliability and efficacy.
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