Detection of Copy Number of Mitochondrial DNA in Iraq Population

Abstract

Mitochondrial DNA copy number (mtDNA-CN), a measure of the number of mitochondrial genomes percell. A cell contains two copies of genomic DNA compared to a single copy of DNA per chromosome. Thequantitative real-time PCR (qPCR) is the gold standard method for measuring mtDNA-CN, the quantificationafter DNA extraction it is an important step, the quantitation of DNA plays a central role inmedical andforensic DNA analysis, which provides the information about the amount of DNA present in unknownsamples. In this study the quantitative results showed that the higher value 74941 and the lower value 190.A multiplex quantitative PCR assay developed to amplify target sequences of different length, which allowsfor the assessment of DNA degradation in samples of forensic interest. The quantification of DNA by qPCRrelies on the detection of amplified product (amplicon) at each cycle of the PCR.