Abstract
The etiologic agent of tetanus, Clostridium tetani, is an obligate anaerobic and spore-forming bacillus; and remains an important public health problem in developing countries. Although, the diagnosis is based mainly on history and presentation of tetanus symptoms. Some recent reports indicate that a patient may not manifest any symptoms of localized or generalized tetanus. However, one of the new approaches for accurate and rapid detection of microbial pathogens is a polymerase chain reaction (PCR) followed by fluorescence detection. In this report, we have described a fluorescent amplification based specific hybridization (FLASH) based PCR assay for detection of C. tetani. The results of the present study indicated that the FLASH based PCR assay appear to be a suitable and rapid method for identification of cultured isolates of C. tetani. Key words: Clostridium tetani, molecular diagnosis, fluorescent amplification based specific hybridization (FLASH) polymerase chain reaction (PCR), fluorescent probe.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
