Abstract
Abstract One mg trypsin/ml is incorporated into an enrichment medium to improve the detection of Clostridium botulinum type E. The procedure was evaluated using smoked whitefish chubs as well as other kinds of smoked fish inoculated with small numbers of spores of C. botulinum types A, B, and E. A greater number of positives was obtained for all smoked fish inoculated with type E spores when this medium was used for enrichment. In addition, by the use of this medium at 26°C for 5 days, proteolytic C. botulinum types A and B can also be detected. Collaborative study is recommended.
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