Abstract
Detection of Classical Swine Fever Virus Antigen and Nucleic Acid on Blood of Experimentally Infected Piglets
Highlights
Classical swine fever (CSF) is a highly contagious viral hemorrhagic disease of domestic pigs causing serious economic losses (Paton and Greiser-Wilke, 2003)
The standard procedure for the detection of Classical swine fever virus (CSFV) infection is based on virus isolation, which is confirmed by the use of antigen detection ELISA and RT-PCR (McGoldrick et al, 1999; De Smit, 2000)
The aim of present study was to detect CSF virus from blood samples of experimentally infected piglets by antigen ELISA and RT-PCR. This will help to confirm the infection of CSFV well before onset of the clinical signs and symptoms and to implement control strategy more effectively
Summary
CSF is a highly contagious viral hemorrhagic disease of domestic pigs causing serious economic losses (Paton and Greiser-Wilke, 2003). During the phase of CSF epidemics, when a large number of samples need to be screened, antigen detection ELISA was found promising (Shannon et al, 1993). A wide variety of samples are suitable for disease diagnosis, which helps in detection of viral antigen in early phase of infection which reduces the time of diagnosis and spread of disease. The aim of present study was to detect CSF virus from blood samples of experimentally infected piglets by antigen ELISA and RT-PCR. This will help to confirm the infection of CSFV well before onset of the clinical signs and symptoms and to implement control strategy more effectively
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