Abstract
Detection of Citrus psorosis virus (CPsV) by direct tissue blot immunoassay (DTBIA) was attempted using different tissues from CPsV-infected sources. Freshly cut surfaces of different plant organs were gently pressed on nitrocellulose membranes, exposed to a CPsV monoclonal antibody (MAb Ps29) conjugated with alkaline phosphatase, and stained with BCIP-NBT Sigma fast. For comparison, some explants were tested also by DAS-ELISA using the same MAb and a polyclonal antiserum for plate coating. Purple staining (positive reaction) was readily observed when flower explants were used, especially the ovary. DTBIA from ovaries correlated with DAS-ELISA, proving reliable and sensitive for the rapid detection of CPsV infections.
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