Abstract
Detection of chronic lymphocytic leukemia subpopulations in peripheral blood by phage ligands of tumor immunoglobulin B cell receptors
Highlights
1234567890();,: 1234567890();,: To the Editor: The B cell receptor (BCR) is an immunoglobulin (Ig) expressed on the membrane surface of mature B cells [1]
The variable sequences of the IgBCR are generated during B-cell differentiation through somatic recombination, so called VDJ recombination, and somatic hypermutation [2]
Since the variable regions are B cell specific, the sequence of IgBCR allows the identification of single B-cell clones [3]
Summary
Being the VH1-69 rearrangement of IgBCR often associated with poor prognosis of CLL [12], we considered the possibility to detect the VH1-69 U-CLL population in peripheral blood using phage p1 as specific ligand To this end, we analysed the immunophenotype and phage binding profile of B cells collected from patient CLL#5 at month 1. The phage p1 detected 73.5% of CD5-positive cells, and the phage p2 and phage p3 detected 86.0% and 81.6%, respectively (Fig. 1g–i) These results were consistent with the occurrence of 75% VH1-69 U-CLL clones in the patient CLL#5 as detected by VDJ sequence (Table 1) and the specific binding of phage p1 to the relative VH1-69 UCLL IgBCR (Fig. 1b).
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