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Abstract
Chloramphenicol (CAP) is a broad spectrum antibiotic that has been banned in many countries due to its serius side effect to human. Detection of CAP residue in food has been determined to a minimum required performance limit (MRPL) of 0.3 ng/g. The purpose of this research was to conduct the analysis of CAP residue in bovine meat by using LCMS and to study the presence of CAP residue in marketed bovine meat samples. LC separation was done on a Shimpack column C18 with ammonium acetate 10 mM/water as mobile phase, and ESI-MS analysis in negative ion mode. The coefficient of determination, R2 = 0.9981 at concentration of 0.125, 0.25, 0.63, 1,00 and 2.00 ng/g. Recovery at three fortification levels (0.25, 0.50 and 1.00 ng/g) was in the range 77.5, 97.3 and 83.4%. The decision limit and the detection capability were 0.15 ng/g and 0.17 ng/g respectively. Analysis results of 52 marketed samples showed that CAP residue were detected in 9 samples in the concentration range of 0.14 to 2.70 ng/g and 6 among those positive samples were above the MRPL value. Therefore, it is important to increase the awareness and also to monitor regularly CAP residues in food originated from animal to provide safe food for the consumers. Key Words : Chloramphenicol, Residue, LCMS, Bovine Meat
Highlights
Chloramphenicol (CAP) is a broad-spectrum antibiotic with historical veterinary uses in all major food-producing animals and with current uses in humans and companion animals
In order to fulfill the minimum required performance limit (MRPL) requirement for CAP detection, this study aimed to demonstrate the ability of LCMS to detect CAP residue in bovine meat at least at the level of 0.3 ppb and to determine the presence of its residue in local meat samples marketed in Indonesia
Structural information obtained from LCMS generated in the mass spectra from protonated or deprotonated molecules resulting from the compound ions combining with the mobile phases
Summary
Chloramphenicol (CAP) is a broad-spectrum antibiotic with historical veterinary uses in all major food-producing animals and with current uses in humans and companion animals. It is effective against both Gram-positive and Gram-negative organisms, rickettsiae, chlamydiae, and mycoplasmas. CAP has shown serius side effect to human, cause bone marrow depression and a severe aplastic anemia (Reeves 2012). The International Agency for Research on Cancer (IARC) in 1990 assigned CAP in group 2A and considered as ‘‘probably carcinogenic to humans’’. Low doses of administered CAP are still able in resulting residues in edible tissues from treated food of animal source.
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