Abstract
This study evaluated the feasibility of using uncoated iron (II, III) oxide nanoparticles (IONP) obtained by electric explosion of wire in air for labelling living mesenchymal stromal cells and their subsequent visualization by magnetic resonance imaging (MRI) using the 1T and 1.5T clinical MRI scanners. The uptake of uncoated IONP by MSC was demonstrated for the wide range of IONP concentration in the cell culture medium. The cells did not change their proliferative activity, viability, and the set of surface markers. Iron oxide nanoparticles obtained by an electric explosion of wire in an atmosphere of air had a shape close to spherical. According to dynamic lateral light scattering, laser diffraction, and transmission electron microscopy, the particle size varied from 14 to 136 nm. Particles up to 136 nm accounted for 75%, while particles less than 36 nm accounted for 10%. A wide range of particle sizes made it possible to select MRI parameters suitable for labelled cells detection in animal tissues in both the T2 and the T1 relaxation mode.
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