Abstract
Sweet potato (Ipomoea batatas) noodles are a traditional Chinese food with a high nutritional value; however, starch adulteration is a big concern. The objective of this study was to develop a reliable method for the rapid detection of cassava (Manihot esculenta) components in sweet potato noodles to protect consumers from commercial adulteration. Five specific Loop-mediated Isothermal Amplification (LAMP) primers targeting the internal transcribed spacer (ITS) of cassava were designed, genomic DNA was extracted, the LAMP reaction system was optimized, and the specificity of the primers was verified with genomic DNA of cassava, Ipomoea batatas, Zea mays, and Solanum tuberosum; the detection limit was determined with a serial dilution of adulterated sweet potato starch with cassava starch, and the real-time LAMP method for the detection of the cassava-derived ingredient in sweet potato noodles was established. The results showed that the real-time LAMP method can accurately and specifically detect the cassava component in sweet potato noodles with a detection limit of 1%. Furthermore, the LAMP assay was validated using commercial sweet potato noodle samples, and results showed that 57.7% of sweet potato noodle products (30/52) from retail markets were adulterated with cassava starch in China. This study provides a promising solution for facilitating the surveillance of the commercial adulteration of sweet potato noodles from retail markets.
Highlights
Sweet potato (Ipomoea batatas) is a dicotyledonous plant that belongs to the family of Convolvulaceae.The roots of sweet potato are starchy, sweet-tasting, and tuberous vegetables
Cassava starch is often incorporated into sweet potato starch during the production of sweet potato noodles; a reliable method for the identification of starch species is critical for the surveillance of commercial adulteration [1]
Since the Loop-mediated isothermal amplification (LAMP) technique was first reported in 2000 [12], researchers have explored its usage for analysis in various fields due to its excellent properties, including rapidity, sensitivity and specificity [16,17]; the reports have been cited more than 5568 times, as listed by Google
Summary
Sweet potato (Ipomoea batatas) is a dicotyledonous plant that belongs to the family of Convolvulaceae. The roots of sweet potato are starchy, sweet-tasting, and tuberous vegetables. Sweet potato noodles are favored by many consumers due to this food’s ease of cooking, good nutritional value, and easy digestion. There is a huge market for this food product in Asia, including China, Japan, and Korea. The food labels of noodle products are required to specify the starch source to prevent adulteration and to protect consumers. The mixture of low-priced starch into high-priced starch is still very common. Cassava starch is often incorporated into sweet potato starch during the production of sweet potato noodles; a reliable method for the identification of starch species is critical for the surveillance of commercial adulteration [1]
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