Abstract
A steady state kinetic method for measuring lactic dehydrogenase (LDH) isoenzymes has been developed which relates the inhibition of heart type LDH to the composition of isoenzyme subunits. The results of LDH inhibition tests indicate that human serum LDH associated with myocardial infarcts is derived from both heart and liver. Analysis of the data indicate that a serum LDH elevation resulting from a myocardial infarction may be classified into two groups, depending on the level of serum LDH and the type of serum LDH. One group represented individuals with heart damage more extensive than individuals in the other group. A statistical treatment of the data with analysis of variance and discriminant analysis supports the findings and conclusions.
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