Detection of C677T, rs75582903 and SNV64878785 in Methylenetetra hydrofolate Reductase Gene (MTHFR) in Meningioma among Sudanese patients

Abstract

Introduction: Meningiomas are commonly benign tumors come from the arachnoid cap cells, and the incidence is account 6 cases per 1000000 of population per year or even much higher in African countries, in Sudan during the period May 2005-May 2012 a total of 405 patients were operated upon and diagnosed as having cranial meningioma, regarding the etiologies of meningiomas are not yet known; it may be related to radiation exposure, genetic disorders (Neurofibromatosis type2), hormonal imbalance (progesterone) and single nucleotide polymorphisms (SNPs).
 Aims of the study: This study aimed to amplify MTHFR gene in specific region using PCR, and to detect C677T, rs755829023 and SNV64878785 of MTHFR gene in meningioma among Sudanese patients.
 Materials and Methods: This was a cross-sectional study conducted at the National Center for Neurological Sciences between May to August 2022, Khartoum Sudan, all meningioma patients attending the national center of neurological sciences during the period of the study was included. Demographic variables were included (age and gender), and the clinical data included (meningioma histology subtype and WHO grading), tissue and blood samples were collected, DNA extraction from the tissue and blood samples was done, polymerase chain reaction was made to amplify specific region of MTHFR gene. PCR products were sent for Sanger sequencing to Macrogen Europe Laboratory. Data was collected and analyzed by using Statistical Package for the Social Sciences (SPSS) version 19, and sequencing was analyzed using, BioEdit and Mutation taster.
 Results: In this study the most affected age group was 41-50 years, female was the most common, WHO grade I meningioma constituted 70% of the cases mostly the fibrous subtype. C>T (g.10369 C>T) was detected in 100% of the cases and deletion C (g.10380-10380delC) was detected only in the cases 16.6%. 
 Conclusion: In this study, female was the most common 11(55%) with male to female ratio 1:2, the most affected age group was ranging from 41-50 years constituted 35%, WHO grade I meningioma was detected in 70% of the patients. C>T (g.10369 C>T) was detected in 100% of the cases and deletion C (g.10380-10380delC) was detected only in the cases 16.6%.